Table 2.
Thirty most prevalent components of the soluble mating secretome.
| Rank | Accession Number | Mol Mass | Avg Tot Spectral Counts | SEM/ Avg |
Description |
|---|---|---|---|---|---|
| 1 | Cre11.g477950.t1.1 | 94 kDa | 189.1 | 0.13 | ADP-ribosylglycohydrolase |
| 2 | Cre02.g088200.t1.2 | 58 kDa | 148.4 | 0.09 | Protein disulfide isomerase 1, RB60 |
| 3 | Cre02.g143200.t1.1 | 122 kDa | 71.7 | 0.05 | Alanine tRNA ligase |
| 4 | Cre02.g080700.t1.2 | 72 kDa | 66.9 | 0.10 | ER associated Hsp70 protein |
| 5 | Cre01.g038400.t1.2 | 47 kDa | 56.4 | 0.15 | Calreticulin 2, calcium-binding protein |
| 6 | Cre14.g633750.t1.1 | 122 kDa | 51.7 | 0.07 | Importin β-3 homolog |
| 7 | Cre06.g298650.t1.2 | 53 kDa | 51.0 | 0.43 | Translation initiation factor 4A |
| 8 | Cre02.g080650.t1.2 | 93 kDa | 41.4 | 0.09 | ER associated heat shock protein 90B |
| 9 | Cre09.g394200.t1.1 | 156 kDa | 37.7 | 0.34 | Flagellar associated protein |
| 10 | Cre01.g034000.t1.2 | 97 kDa | 37.3 | 0.26 | Importin β |
| 11 | Cre14.g620600.t1.2 | 52 kDa | 34.7 | 0.24 | Pherophorin, PHC2 |
| 12 | Cre09.g406600.t1.1 | 38 kDa | 33.7 | 0.35 | ChlamyFPv5, 2 KCl peptides |
| 13 | Cre06.g258800.t1.1 | 132 kDa | 26.7 | 0.24 | OH-Pro-rich glycoprotein, GP2 (FAP3) |
| 14 | Cre10.g431800.t1.2 | 70 kDa | 25.2 | 0.37 | Arylsulfatase |
| 15 | Cre07.g330200.t1.2 | 30 kDa | 23.2 | 0.12 | Radial spoke protein 9 |
| 16 | Cre02.g089500.t1.2 | 43 kDa | 22.1 | 0.21 | Proline rich extensin signature |
| 17 | Cre03.g144564.t1.1 | 81 kDa | 22.0 | 0.30 | Matrix metalloproteinase, MMP13 |
| 18 | Cre07.g321400.t1.1 | 199 kDa | 20.5 | 0.13 | Flagellar associated protein |
| 19 | Cre02.g089450.t1.2 | 38 kDa | 20.4 | 0.33 | Proline rich extensin signature, HRP5 |
| 20 | Cre02.g077850.t1.2 | 83 kDa | 18.6 | 0.34 | Flagellar associated protein, FAP212 |
| 21 | Cre09.g407700.t1.2 | 54 kDa | 17.3 | 0.08 | Cysteine endopeptidase, CEP1 |
| 22 | Cre09.g401900.t1.2 | 132 kDa | 16.9 | 0.37 | Proline rich extensin signature |
| 23 | Cre12.g487700.t1.2 | 77 kDa | 15.9 | 0.13 | Serine/threonine protein kinase |
| 24 | Cre09.g393700.t1.1 | 71 kDa | 15.7 | 0.16 | Matrix metalloproteinase, MMP3 |
| 25 | Cre11.g467547.t1.1 | 83 kDa | 13.0 | 0.26 | Glutamyl/glutaminyl-tRNA synthetase |
| 26 | Cre02.g077800.t1.2 | 86 kDa | 12.5 | 0.45 | Proline rich extensin signature (FAP310) |
| 27 | Cre02.g102050.t1.1 | 92 kDa | 12.4 | 0.20 | Proline rich extensin signature (FAP328) |
| 28 | Cre11.g479250.t1.2 | 54 kDa | 12.1 | 0.11 | Ran GTPase-activating protein, |
| 29 | Cre06.g304500.t1.2 | 40 kDa | 10.9 | 0.18 | Zygote-specific protein |
| 30 | Cre12.g533100.t1.1 | 21 kDa | 10.6 | 0.41 | CHRD domain, PF07452 |
The signal peptide-containing proteins identified in the merged dataset were sorted by average total spectral counts (Avg Tot Spectral Counts); the entire list, grouped by function, is provided in Supplemental Table S4. The 30 most prevalent proteins, which account for 72% of the total spectral counts, are shown here.