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Adipocyte-derived mesenchymal stem cells (hAD-MSCs) transduction with lentiviral particles. (a) hAD-MSCs before transduction. (b) hAD-MSCs transduced with pCDH-RIL-23R-PGK-copGFP lentiviral vector. (c) Transduced hAD-MSCs after puromycin selection; abundant green cells and GFP expression exhibit high transduction level (d) FACS analysis of the transduction rate after puromycin selection indicated that more than 90% of the cells were GFP positive.
