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Primer sets used for RT-PCR.
| Gene | Primer sequence (5′ → 3′) | Product size (bp) |
|---|---|---|
| c-Myc | Forward: TCGGATTCTCTGCTCTCCTC | 413 |
| Reverse: CGCCTCTTGACATTCTCCTC | ||
|
| ||
| 4-Oct | Forward: GACAACAATGAGAACCTTCAGGAGA | 218 |
| Reverse: TTCTGGCGCCGGTTACAGAACCA | ||
|
| ||
| SOX2 | Forward: AACCAAGACGCTCATGAAGAAG | 341 |
| Reverse: GCGAGTAGGACATGCTGTAGGT | ||
|
| ||
| NANOG | Forward: ATAGCAATGGTGTGACGCAG | 219 |
| Reverse: GATTGTTCCAGGATTGGGTG | ||
|
| ||
| GAPDH | Forward: GTGGTCTCCTCTGACTTCAACA | 210 |
| Reverse: CTCTTCCTCTTGTGCTCTTGCT | ||
