Table 1.

Representative quantitative data for select compounds identified through SMM screening of an RNA of interest (RNA) and Control RNA (RNA_ctrl) compared with a buffer only control (buffer)

Compound	Zbuffer	ZRNA	ZRNActrl	CVRNA	Hit	Notes
1	0.14	8.34	1.67	10.5	Yes	Specific binder
2	0.54	10.3	0.76	23.8	No*	Specific binder ruled out by structure (e.g., polycation)
3	0.99	10.0	7.37	10.1	No	Nonspecific binder
4	0.03	1.26	0.97	0.91	Yes	False negative
5	0.22	6.69	−0.11	146	Noa	False positive (e.g., dust particulate)
6	13.0	3.03	4.15	3.8	No	Autofluorescent compound

^aThese compounds mathematically satisfy the defined hit criteria, but were later ruled out upon visual inspection or based on structural features