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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Behav Brain Res. 2018 Nov 14;359:386–395. doi: 10.1016/j.bbr.2018.11.018

Figure 5.

Figure 5

Behavioral design (A) and data (B, C), and post-context-preexposure IEG expression in the mPFC (D) and dHPC (E) for the HC, EtOH, and SI experimental groups. (A) Animals were given alcohol or sham-intubation from PD4–9, and then run through the full three-day CPFE procedure from PD31–33. Littermates of this behavior group were sacrificed 30min after context exposure and IEG mRNA expression in the mPFC and dHPC was assayed via qPCR. (B, C) The SI-Pre group froze significantly higher than the EtOH-Pre group and both Alt-Pre control groups during the 3min post-shock and 5min retention freezing tests (ps < .001). (D) The SI group had significantly higher expression of every IEG above both the EtOH and baseline HC control group (ps > .001). The EtOH group had significantly higher c-Fos, Arc, and Npas4 expression than the HC group (ps > .001). (E) Both SI and EtOH groups had significantly higher expression of c-Fos, Arc, and Npas4 above HC control levels, with no difference between the two dosing groups (ps > .18). # indicates significant elevation above the HC group; * indicates a significant difference between SI and EtOH group