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VEGF media supplementation stimulates angiogenesis. The mouse mesometrium tissues were cultured with MEM (A) or with MEM supplemented with VEGF (B) for 5 days. This resulted in capillary sprouts (arrows), and the presence of vascular islands (arrowheads) which are defined as disconnected endothelial segments. The angiogenic response was quantified by counting the total number of sprouts per total vascular length (C). Data is shown as the mean + SEM and ** represents p < 0.005.
