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. 2018 Dec;59(15):6057–6066. doi: 10.1167/iovs.18-24828

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Copyright 2018 The Authors

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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Recombinant grn1 induces retinal regeneration by the induction of retinal regeneration–related genes. (A) Intravitreal injection of recombinant grn1 induced BrdU-positive cells without retinal injury. BrdU was injected at 4 dpi. (B) Quantitative analysis of BrdU-positive cells on recombinant grn1 injection. The number of BrdU-positive cells was counted in a single section, including the optic nerve. (C) RT-PCR showing the induction of retinal regeneration–related genes by recombinant grn1 injection (200 ng). The RNA samples were obtained at 1 dpi of recombinant grn1. (D) Quantitative PCR shows recombinant grn1 highly induced socs3, ascl1a, and lin28, and weakly induced pax6 and c-mycb. (E) BrdU-positive cells have migrated to various retinal cell layers at 14 dpi of recombinant grn1 (200 ng). Data are the means ± SEMs; n = 3. P < 0.05 versus vehicle-treated group. N.S., not significant. Scale bar: 200 μm. The cropped blots are used in this figure and the full-length blots are presented in Supplementary Figure S7.