Figure 1.

High-antibody titers produced by immunization of TgElk mice with monomeric or dimeric recombinant PrP. A, TgElk mice were immunized with four different immunogens at 3-week intervals five times (one priming and four booster doses), and blood sampling was performed either before starting vaccination or 10 days after the fourth booster dose. The animals were i.p. inoculated at day 99 with 1% brain homogenate (BH) from a terminally ill CWD-infected TgElk mouse, and the animals received two more booster doses post-inoculation at 6-week intervals. B, antibody titers using end-point ELISA from the four vaccinated groups. Mice were vaccinated with Mmo, Dmo, Mdi, or Ddi recombinant PrPs, and CpG was used as adjuvant for all groups. The antibody titer for each individual mouse was determined by end-point dilution. The y axis indicates the serum fold dilution. The cutoff was calculated as three times average OD (405 nm) of preimmune sera.