Figure 2.

Expression of Fam210b is enriched in vertebrate tissues with high heme content. A, Fam210b expression (red pseudocolor) is enriched in the murine fetal liver (FL) at E12.5. B, in murine tissues, Fam210b expression is enriched in the bone marrow and fetal liver (erythroid), adult liver, skeletal muscle, and testis, all of which require high levels of iron for their function. C, zebrafish fam210b is maternally expressed during early embryonic development. At 24 hpf, fam210b expression is enriched in neural tissue and the intermediate cell mass, the site of primitive hematopoiesis (top). The expression of fam210b in the ICM is abolished in cloche embryos, which do not form hematopoietic or vascular tissue. In contrast, fam210b expression in the intermediate cell mass is expanded in dino embryos, which exhibit a ventralized phenotype (bottom), paralleling the increased expression of erythroid gata1. D, at 72 hpf, fam210b expression persists in the neural tissue and is enriched in the liver, delineated by sid4 expression. n = 3.