Table 1.
Binding affinity measurements with two receptor glycoforms and three IgG1 Fc glycoforms determined by surface plasmon resonance
| Receptor | IgG1 Fc G0F |
IgG1 Fc G2F |
IgG1 Fc G0 |
|||
|---|---|---|---|---|---|---|
| KD (nm) | ± error | KD (nm) | ± error | KD (nm) | ± error | |
| rCD16a-CT | 10 | 70 | 2 | 25 | 1 | |
| rCD16a G129D-CT | 22,000 | 1,000 | 12,500 | 600 | 3,000 | 200 |
| rCD16b-CT | 6,400 | 400 | 4,200 | 300 | 950 | 100 |
| rCD16b D129G-CT | 100 | 10 | 48 | 3 | 8 | 1 |
| rCD16a-Man5 | 48 | 2 | 22 | 1 | 3.9a | 1.0 |
| rCD16a G129D-Man5 | 9,500 | 300 | 4,000 | 200 | 1,100 | 100 |
| rCD16b-Man5 | 5,200 | 300 | 2,900 | 200 | 560 | 30 |
| rCD16b D129G-Man5 | 13 | 1 | 6.0 | 0.3 | 3.6a | 1.0 |
| rCD16b | ||||||
| N38Q/N64Q/N74Q/N169Q-Man5 | 6,400 | 600 | 4,000 | 400 | 650 | 50 |
a KD values were calculated from kinetic data.