Table 2.
Association and dissociation rate constants measured from kinetic fits of the surface plasmon resonance sensorgrams
Receptor | IgG1 Fc G0F |
IgG1 Fc G0 |
||||||||
---|---|---|---|---|---|---|---|---|---|---|
kON (mm−1s−1) | ± error | kOFF (s−1) | ± error | KD (nm) | kON (mm−1s−1) | ± error | kOFF (s−1) | ± error | KD (nm) | |
rCD16a-CT | 39 | 11% | 0.00506 | 14% | 130 | 98 | 13% | 0.00420 | 10% | 43 |
rCD16b D129G-CT | 306 | 18% | 0.01853 | 16% | 61 | 317 | 4% | 0.00323 | 10% | 100 |
rCD16a-Man5 | 437 | 19% | 0.01729 | 16% | 40 | 625 | 1% | 0.00242 | 29% | 3.9 |
rCD16b D129G-Man5 | 422 | 2% | 0.00440 | 18% | 10 | 685 | 4% | 0.00246 | 20% | 3.6 |