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Production of soluble Siglec-14 is not proportional to the expression level of membrane-bound Siglec-14. A, ELISA of soluble Siglec-14 produced by FLAG-Siglec-14/THP-1 and U937 cell lines. Siglec-14–specific mouse mAb (clone 40-1) was used for detection. B, expression levels of membrane-bound Siglec-14 on FLAG-Siglec-14/THP-1 and U937 cell lines. Solid line, cells stained with anti-Siglec-14 antibody (clone 40-1); gray-shaded area, cells stained with control mouse antibody.
