Figure 2.

Human bone marrow cells produce alternatively spliced mRNA encoding soluble Siglec-14. Human bone marrow first-strand cDNA library (A–C) and first-strand cDNA prepared from U937 cells (C) were used as templates. A, agarose gel electrophoresis of 3′RACE PCR products obtained using a gene-specific primer (annealing to exon 5) and a universal primer. Products of nested PCR (second round) were separated by agarose gel electrophoresis. B, agarose gel electrophoresis of RT-PCR products with the primer pair flanking intron 5. C, relative abundances of membrane-bound (solid bars) and soluble (open bars) SIGLEC14 mRNA isoforms, normalized against ACTB mRNA.