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LPE acylation assays in LplTKp spheroplasts. A, TLC images showing conversion of LPE to PE ± 1% Triton X-100 mediated by LplTKp WT and mutants. E. coli ΔlplT harboring empty vector served as (−) control. B, the relative LPE acylation activity (% of WT) of mutants. C, Western blotting of LplTKp WT and mutant proteins extracted from spheroplasts and developed using anti-His antibody. The same amount of total protein was loaded in each lane.
