Table 1.
Phospholipid compositions of spheroplasts generated from E. coli expressing LplTKp WT or mutants
| Cardiolipin | PE | PG | LPE | LPG | |
|---|---|---|---|---|---|
| BL21(DE3) | 9.0 | 70.7 | 20.3 | NDa | ND |
| ΔlplT | 7.0 | 55.5 | 16.2 | 16.6 | 4.7 |
| LplTKp WT | 8.3 | 71.2 | 20.4 | ND | ND |
| D30N | 7.0 | 54.7 | 16.2 | 16.7 | 5.4 |
| K120C | 7.5 | 57.3 | 16.5 | 14.6 | 4.1 |
| R236M | 7.0 | 55.8 | 16.5 | 16.4 | 4.3 |
| E351C | 7.5 | 58.0 | 16.7 | 14.1 | 3.7 |
| N352C | 7.3 | 57.3 | 15.4 | 15.9 | 4.0 |
| N31C | 6.7 | 57.1 | 13.7 | 17.9 | 4.7 |
| N137C | 8.7 | 71.3 | 20.0 | ND | ND |
| L34F | 6.8 | 54.9 | 16.7 | 14.0 | 7.7 |
| F35N | 7.4 | 54.0 | 14.8 | 15.3 | 8.5 |
| L38F | 8.3 | 55.2 | 16.9 | 13.7 | 6.0 |
| I148F | 7.5 | 64.2 | 19.2 | 6.9 | 2.3 |
a ND, not determined.