Figure 1.
MLIP expression in the heart and brain of Mlip+/+ and MlipΔE1/ΔE1 mice. A, generation of Mlip knockout mouse model (Mlip ΔE1) by deletion of exon 1 (E1) of the Mlip gene and its proximal promoter. Orange arrows indicate flox sites. Mlip Ab, polyclonal antibody targeting an epitope in exon 11 of the gene. B, Western blot with MLIP antibody of Mlip+/+ and MlipΔE1/ΔE1 brain and heart. The polyclonal MLIP antibodies are specific for an epitope encoded within exon 11. Lamin B was used as a loading control. C, PCR amplification of Mlip cDNA from brain and heart of Mlip+/+ and MlipΔE1/ΔE1 mice using forward primers specific for Mlip exon 1b or exon 1a, respectively, and exon 14 reverse primer. Ubc was used as a loading control.