Figure 2.

Identification of two Mlip alternative transcriptional start sites. A, 5′-RACE reactions targeting Mlip were performed on mRNA extracted from adult mouse heart, brain, and skeletal muscle and subsequently identified by DNA sequencing. B, tissue distribution of exon 1a and exon 1b identified from 96 clones from heart, brain, and skeletal muscle. C and D, display of UCSC Genome Browser (ENCODE project data) showing histone H3 epigenetic marks (H3K27Ac, H3K4Me1, and H3K4Me3; the height of the peak correlates with the strength of signal in a given cell line) centered on exon 1a (C) and exon 1b (D) of the Mlip gene in different cell lines.