. 2018 Oct 25;293(51):19909–19918. doi: 10.1074/jbc.RA118.005485

Table 2.

Cloning and mutagenesis primer sequences

	Cloning and primer sequences (5′-3′)
Cloning primers
APK270pet28aFWD	`ATACAGCTCGAGTCAGGTCAGGCGAAGTCCGTCGGAGG`
APK270pet28aREV	`TTAGATCATATGGGCGTGATCACCGACGATGTCACCATC`
APK780Δ1–23pet28aFWD	`CGAAGCTAGCATGCCGGGACTTCCAGCTGACCACGAACTG`
APK780Δ1–23pet28aREV	`GCTAAAGCTTCTATGCTGTCGGGGTGAGGGGTGACGCAAA`
Mutagenesis primers
E33AFWD	`AACCTGTCCGCGAACGCGACC`
E33AREV	`GGTCGCGTTCGCGGACAGGTT`
D235AFWD	`GATTTCGATGCCTGCGGATTC`
D235AREV	`GAATCCGCAGGCATCGAAATC`
R213AFWD	`GCCGACCTGGCGTTGGCCAAC`
R213AREV	`GTTGGCCAACGCCAGGTCGGC`
R290AFWD	`GTGTTCCTGGCCAGGCTCTTG`
R290AREV	`CAAGAGCCTGGCCAGGAACAC`