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. 2018 Oct 29;293(51):19624–19632. doi: 10.1074/jbc.RA118.003720

Figure 4.

Figure 4.

Effect of HuR and HuD on Kv11.1 isoform expression. A, structure of the short KCNH2 gene construct and a schematic presentation of the RPA protocol for Kv11.1a and Kv11.1a-USO. B, RPA analysis of mRNA from Flp-In HEK293 cells stably expressing the short KCNH2 gene following transfection with vector control, HuR, or HuD. C, histogram showing modulation of Kv11.1a (1a) and Kv11.1a-USO (1a-USO) transcripts following HuR and HuD overexpression. RPA signals were quantified and shown as an isoform percentage of the total signal (1a + 1a-USO). The overexpression of HuR or HuD resulted in significantly increased expression of Kv11.1a transcripts and significantly decreased expression of Kv11.1a-USO transcripts compared with vector-transfected control (*, p < 0.05, n = 3, error bars, S.E.).