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. 2018 Dec 28;12(3):513–522. doi: 10.1016/j.tranon.2018.12.004

Figure 2.

Figure 2

CBD and TMZ modulate EV release from GBM cells. EV release was assessed by NTA analysis after 1 h treatment with CBD (5 μM), TMZ (800 μM) and combinatory treatment of CBD (5 μM) with TMZ (800 μM). A) In LN18 cells, CBD significantly reduced exosome release. B) In LN18 cells, the release of smaller MVs was significantly reduced by CBD and TMZ but not after combinatory treatment, compared to DMSO controls. C) In LN18 cells, larger MVs were significantly reduced by CBD and CBD-TMZ combinatory treatment. D) In LN229 cells, exosome release was significantly increased in single CBD or TMZ treatment, as well as in the CBD-TMZ combinatory treatment, compared to control DMSO treated cells. E) In LN229 cells, the release of smaller MVs was increased in all treatment groups, compared to DMSO control-treated cells. F) In LN229 cells, the release of larger MVs was increased in all treatment groups, compared to DMSO controls. The P values indicated above the bars in the histograms represent significant changes compared to DMSO control; significant changes for TMZ versus combinatory treatment of TMZ with CBD are also indicated.