Figure 4.

GG-NER complex adjacent nucleosomes are established and remodeled following UV irradiation in a Rad16-dependent fashion. (A) MNase-seq data of wild-type and rad16 mutant cells were used to plot cumulative nucleosome positions around GCBSs (n = 2664) in the absence of UV irradiation. The annotation of the nearest gene was used to infer strand information to align these genomic positions according to gene orientation as indicated by the arrows on the x-axis depicting the relative direction the GCBS and ORF. The x-axis denotes 2-kbp regions surrounding the GCBSs, while the y-axis indicates nucleosome occupancy as measured by normalized reads. (B) As described in A, but showing UV-induced changes to nucleosome positions around GCBSs and accompanying ORFs in rad16-mutated, GG-NER–defective cells.