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. Author manuscript; available in PMC: 2019 Oct 10.
Published in final edited form as: Neuron. 2018 Oct 10;100(1):120–134.e6. doi: 10.1016/j.neuron.2018.09.017

Figure 8. CD47 Is Required for Activity-Dependent Microglial Engulfment of Weaker RGC Inputs.

Figure 8

(A) Schematic demonstrating the experimental paradigm.

(B and C) Representative three-dimensional reconstructions of P5 WT (B) and CD47KO (C) microglia (green) with internalized CTB-labeled RGC inputs (red and blue) from either the TTX-treated or the saline-treated eye. In the WT microglial cell, there are more engulfed inputs from the TTX-treated eye, but in the CD47KO microglial cell, a similar amount of engulfed material from both eyes is present. Grid line increments = 5 μm.

(D) Graph showing that microglia in the dLGN of WT mice engulfed more CTB-labeled material (volume engulfed CTB/volume of the cell) from the TTX-treated eye than the saline-treated eye, n = 9. ∗p < 0.02, paired t test.

(E) Graph showing that microglia in the dLGN of CD47KO mice engulfed a similar amount of CTB-labeled material from the TTX-treated eye as they did from the saline-treated eye, n = 6. NS, paired t test.