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. Author manuscript; available in PMC: 2019 Jan 2.
Published in final edited form as: Vaccine. 2017 Jul 31;35(37):4997–5005. doi: 10.1016/j.vaccine.2017.07.071

Fig. 3.

Fig. 3.

B cell populations in the spleens and peritoneal cavities of IgA+/+ and IgA−/− mice. (A) Splenic and peritoneal B cells from IgA+/+ and IgA−/− mice were characterized by flow cytometry based on expression of surface CD5, CD11b, and B220. Representative plots showing sequential flow cytometric gating strategies used to identify B-1a (B220+CD11b+CD5+), B-1b (B220+CD11b+CD5lo-neg), and B-2 (B220+CD11b−CD5−) cell populations. (B and C) Numbers of splenic (B) and peritoneal (C) B cell populations in naïve mice. Bars represent the means ± SD from 4–5 mice per group. Panels A-C are representative of four independent and similar experiments.