Table I.
Summary of observed metabolic products of human sperm after incubating with 13C labelled substrates.
| Number of spectra the metabolic product was observed in | |||
|---|---|---|---|
| Incubated substrate | Lactate produced | Bicarbonate produced | Other metabolic products |
| 13Cu-glucose | 8 | 0 | – |
| 13Cu-fructose | 8 | 0 | – |
| 13C1-pyruvate | 8 | 0 | – |
| 13C3-lactate | N/A | 2 | Pyruvate (7 of 8), acetate (6 of 8) |
| 13C2,4-D-3-hydroxybutyrate | 0 | 2 | Acetoacetate (8) |
| 13C1-butyrate | 0 | 1 | Glutamate (2) |
| 13C5-glutamate | 0 | 2 | – |
| 13C2-glycine | 0 | 1 | – |
| 13Cu-galactose | 0 | 0 | – |
Eight incubations were performed for each substrate at 37°C for 24 h with samples subsequently stored at −80°C prior to MRS analysis.