Fig. 3.

Effect of LDLR upregulation on LDL-mediated PCSK9 uptake inhibition. A: Relative uptake, as measured by luminescence assay, of PCSK9-NLuc by HepG2 cells in the presence of increasing concentrations of LDL, and stratified by the presence (blue) or absence (cyan) of sterol-starvation. Note that, in this experiment, LDL was preincubated with PCSK9-NLuc prior to coincubation with the cells. Raw luminescence data are normalized to 1 for PCSK9-NLuc treatment in the absence of LDL and presence of sterol-starvation, and 0 in the absence of PCSK9-NLuc uptake. B: Same experiment (and source data) as in A, but each medium condition is normalized separately to 1 (in the absence of LDL) to highlight the relative impact of LDL under each.