TABLE 2.

Composition of Hex-Cer from ARPE19 and ARPE19-ASAH1 cells

Hex-Cer Species	ARPE19		ARPE19-ASAH1
	Veh	H2O2 (3 h)	Veh	H2O2 (3 h)
C14:0	3.75 ± 0.48	3.44 ± 0.45	2.44 ± 0.24a	2.62 ± 0.11
C16:0	30.76 ± 0.77	32.56 ± 1.61	16.50 ± 1.21b	22.57 ± 0.92c,d
C18:1	0.58 ± 0.06	0.72 ± 0.11	0.31 ± 0.03d	0.57 ± 0.07
C18:0	3.15 ± 0.09	4.02 ± 0.15c	1.75 ± 0.09b	3.52 ± 0.61
C20:0	0.96 ± 0.09	1.04 ± 0.05	0.47 ± 0.05d	0.77 ± 0.05c,d
C22:0	12.57 ± 0.29	23.31 ± 1.85c	7.67 ± 0.48b	15.93 ± 1.10a,e
C24:1	11.25 ± 0.41	23.85 ± 1.01e	5.44 ± 0.34b	17.09 ± 1.22d,e
C24:0	43.73 ± 1.25	48.75 ± 2.99	21.66 ± 0.42b	40.07 ± 0.51a,e
C26:1	2.91 ± 0.14	3.32 ± 0.39	1.82 ± 0.26a	2.62 ± 0.11
C26:0	5.79 ± 1.45	2.21 ± 0.29	3.85 ± 0.69	1.06 ± 0.24a,c
Total	115.44 ± 3.23	143.22 ± 6.65c	61.89 ± 0.68b	106.81 ± 2.45d,e

Cells were treated with Veh or H₂O₂ for 3 h. Absolute quantification (pmol × 1,000) values (mean ± SEM) presented for each species after being normalized with SM.

^aP < 0.05 between ARPE19 and ARPE19-ASAH1 cells of same treatment group; n = 4/condition.

^bP < 0.001 between ARPE19 and ARPE19-ASAH1 cells of same treatment group; n = 4/condition.

^cP < 0.01 between Veh and H₂O₂ treatment; n = 4/condition.

^dP < 0.01 between ARPE19 and ARPE19-ASAH1 cells of same treatment group; n = 4/condition.

^eP < 0.001 between Veh and H₂O₂ treatment; n = 4/condition.