Fig. 6.

Incomplete redundancy in the ability of DGAT1 and DGAT2 to support ApoB secretion in HepG2 cells. HepG2 cells were incubated with oleic acid and glucose as substrates, as described in Methods. ApoB secretion into the medium was measured (using ELISA) for a period of 4 h in the absence (control) or presence of specific inhibitors of DGAT1 (iDGATl) and/or DGAT2 (iDGAT2) as indicated. The rate of ApoB secretion in control cells was 86.26 ng ApoB/h/mg cell protein. Values are expressed as a percentage of the rate observed for control cells in the same experiment for n = 3 separate determinations. Each apoB measurement was performed in duplicate. * P < 0.02 (values that are significantly different from control).