Fig. 3.

LCSFAs palmitate and stearate induce dose-dependent reductions in anterograde mitochondrial trafficking velocity in mouse DRG neurons. A, B: Laurate and myristate have no effect on anterograde mitochondrial velocity. Palmitate treatments exhibit a trending dose-dependent decrease in anterograde velocity (C), whereas stearate treatments ranging from 31.25 to 250 μM induce significant dose-dependent reductions in anterograde mitochondrial trafficking velocity after 24 h (D). The number in each bar is the total number of DRG neurons that were evaluated for each treatment condition in three separate experimental trials. Values are expressed as mean ± SEM. * P < 0.01, ordinary one-way ANOVA with Tukey’s multiple-comparisons test.