LCSFAs palmitate and stearate alter mitochondrial morphology in mouse DRG neurons. Mitochondrial circularity was not significantly affected by 250 μM laurate or 250 μM myristate (A, B), but a significant increase in circularity was observed in 250 μM palmitate and 250 μM stearate-treated DRG neurons (C, D). E, F: The distribution of mitochondrial size in DRG neurons treated with 250 μM laurate and myristate matched the mitochondrial size profile of the 0.25% BSA control. G, H: Treatment with 250 μM palmitate or 250 μM stearate induced a shift in the mitochondrial size profile, resulting in larger mitochondria relative to the 0.25% BSA control. The number of DRG neurons analyzed for changes in mitochondrial morphology are listed in the box plot of each condition. Values are expressed as mean ± SEM. * P < 0.05, unpaired t-test for mitochondrial circularity and Kolmogorov-Smirnov test for mitochondrial size distribution.