TABLE 1.
Lipid composition of exosomes released by individual cell types
| Lipids | PC-3 Cells (36) | PC-3 Cells + HG (38) | Oli-neu Cells (30) | HepG2/C3a (39) | B-Lymphocytes (40) | Mast Cells (41) | Dendritic Cells (41) | Reticulocytes (42) | Platelets (45) | Adipocytes (46) | ||||||||
| % | Factor | % | Factor | %a | Factor | %a | Factor | % | Factor | %b | Factor | %b,c | Factor | % | Factor | % | %a | |
| CHOL | 43.5 | 2.3 | 59 | 1.7 | 43 | 2.3 | 43 | 1.9 | 42.1 | 3.0 | 15 | 1.0 | NR | NR | 47 | 1.03 | 42.5 | 43 |
| SM | 16.3 | 2.4 | 9.1 | 2.0 | 8.2 | 1.5 | 9.7 | 10.8 | 23.0 | 2.3d | 12 | 2.8 | 20 | 2.2 | 8.4d | 1.31 | 12.5 | 12.5 |
| PC | 15.3 | 0.31 | 10.8 | 0.33 | 26.7 | 0.67 | 20 | 0.67 | (20.3)e | (0.76)e | 28 | 0.66 | 26 | 0.6 | 23.5 | 1.03 | 15.9 | 33 |
| PS | 11.7 | 2.1 | 6.9 | 1.2 | 14.9 | 3.0 | 15.6 | 2.4 | (20.3)e | (0.76)e | (16)e | (1.2)e | (19)e | (1.6)e | 5.9 | 0.92 | 10.5 | 1.1 |
| PE | 5.8 | 0.55 | 1.1 | 0.21 | 10.9 | 1.0 | 7.4 | 1.2 | (14.6)e | (0.7)e | 24 | 1.08 | 26 | 1.13 | 12.7 | 0.84 | 3.1 | 4.0 |
| PE ethers | 3.3 | 1.2 | 4.7 | 0.81 | (14.6)e | (0.7)e | 3.2 | |||||||||||
| DAG | 1.5 | 1.5 | 1.1 | 0.92 | 0.8 | |||||||||||||
| PC ethers | 0.81 | 0.40 | 0.7 | 0.28 | 1.4 | |||||||||||||
| PG | 0.17 | 0.17 | 0.1 | 0.07 | NR | |||||||||||||
| PA | 0.16 | 1.8 | 0.1 | 0.33 | (20.3)e | (0.76)e | NR | |||||||||||
| PI | 0.13 | 0.13 | 0.3 | 0.16 | NR | 4.1 | 0.18 | (20.3)e | (0.76)e | (16)e | (1.2)e | (19)e | (1.6)e | 2.4 | 1.1 | 5.2 | 2.3 | |
| Cer | 0.32 | 1.3 | 0.7 | 1.2 | NR | 3.3 | 0.63 | 2.0 | 0.40 | 0.2 | ||||||||
| HexCer | 0.76 | 3.8 | 2.3 | 2.1 | 2.0 | 0.02 | ||||||||||||
| LacCer | 0.12 | 3.0f | 0.7 | 1.8 | NR | |||||||||||||
| Lipid analysis | MS | MS | MS | NR | MS/GC | TLC | TLC/GLC | TLC/GLC | TLC | MS | MS | |||||||
| Exosome preparationsg | SFM + SUC | SFM + SUC | SFM + SUC + SG | uFCS + SUC + IG | uFCS+ SUC+ SG + immunocapture | uFCS + SUC | uFCS + SUC | uFCS + SUC | SUC + IG | SFM + SUC | ||||||||
%, percent of total lipid quantified; Factor, factor of enrichment from cells to exosomes; DAG, diacylglycerol; NR, not reported; LacCer, lactosylceramide; PA, phosphatidic acid; PG, phosphatidylglycerol; SFM, serum free medium; uFCS, ultracentrifuged FCS; SUC, sequential centrifugation; SG, sucrose gradient; IG, iodixanol gradient.
Percent CHOL not reported; CHOL set to 43% to better compare the content of other lipid classes with the other data shown.
Recalculated from their data.
CHOL not reported; the sum for the other lipid classes is 100% (including LysoPC not included in this table).
Sum of SM and the glycosphingolipid, GM3.
Sum for all classes shown in parentheses and having the same numbers.
Enrichments of other lipid classes are shown in Fig. 2.
Exosome preparations: methods used to isolate the exosome preparations.