Table 1.
Summary of biochemical data on PGM1 loop mutants
| Name | Source mutation | Sol. protein (%) |
MW (kD) |
Polydispersity (%) |
Rh (nm) |
T0.5 (°) |
kcat (1/s) |
Km (μM) |
kcat / Km (μM/s) |
Phosphorylation before/after G16P (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| WTa | - | 90 | 67 | 20 | 3.7 | 46.3 | 143+/−2 | 80 +/−4 | 1.8+/−0.1 | 35 / 90 |
| R503A | in-house | 90 | 63 | 21 | 3.8 | n.d. | n.c. | n.c. | n.c. | 40 / 85 |
| R503Qb | patient | 90 | 72 | 0 | 3.8 | 48.7 | n.c. | n.c. | n.c. | 25 / 92 |
| G508R | ExAC | 80 | 10 2 |
18 | 4.3 | - | - | - | - | 34 / 79 |
| G511R | ExAC, Cosmic |
80 | 22 2 |
0 | 6.0 | - | - | - | - | 13 / 82 |
| R515A | in-house | 90 | 51 | 19 | 3.3 | n.d. | n.c. | n.c. | n.c. | 31 / 87 |
| R515Lb | patient | 75 | 68 | 15 | 3.7 | 46.8 | n.c. | n.c. | n.c. | 33 / 49 |
| R515Q | Cosmic | 90 | 53 | 19 | 3.3 | 47.6 | n.c. | n.c. | n.c. | 34 / 82 |
| R515W | ExAC, Cosmic | 70 | 66 | 16 | 3.6 | 44.9 | n.c. | n.c. | n.c. | 17 / 36 |
Abbreviations: n.c. – not characterizable; n.d. – not done; (−) unable to assess due to aggregation;
ExAC: Exome Aggregation Consortium (Lek et al., 2016); Cosmic: Catalog of Somatic Mutations in Cancer (Forbes et al., 2017).
a
Data for WT PGM1 previous published (Lee et al., 2014) except for T (this study).
b
0.5 Kinetic data previously published (Wong et al., 2015).
Phosphorylation assessed via electrospray ionization mass spectrometry as described in Methods.