Figure 1.
Hypoxia reduced the sensitivity of SKOV3 cells to cisplatin (mean ± SD, n=6, %).
Notes: SKOV3 cells were exposed in hypoxia (1% O2, closed line) or normoxia (opened line) chamber, the supernatant were collected to stimulate another cells in normoxia for 24 hours. Then the cells were treated with cisplatin (3 µg/mL) for 24 hours, the viability was measured using CCK8 assay kit by fluorescence microplate reader and inhibitive rate of cisplatin was calculated. (A) The inhibition rates of cisplatin to SKOV3 in hypoxia; (B) the HICR value of cisplatin to SKOV3 in hypoxia; (C) the inhibition rates of cisplatin to SKOV3 after treated with supernatant from hypoxia SKOV3 cells for different times. *P<0.05, **P<0.01, compare with the normoxia SKOV3 cells at the same time and the same volume supernatant.
Abbreviations: HICR, hypoxia-induced chemoresistance; v/v, volume per volume.