Figure 3.

ROC-P method. (A) ROC curves for individual assays. The x-axis represents percentiles of the combined postdose values, and the y-axis is the percentage of postvaccination levels greater than or equal to the corresponding percentile threshold. The ROC curve is shown for each dose arm alone and for the combination of all vaccine formulations; (B) Three-dimensional ROC surface plot for the combination of results from the MN and ELISPOT assays compared to postdose values from all arms combined. The x-axis and y-axis are percentiles of the combined postdose distribution for ELISPOT and MN values, respectively, and the z-axis is the percentage of postvaccination responses greater to or equal than both the MN and ELISPOT thresholds. The ROC surface is shown for each formulation and for all formulations combined. The ROC surface for the all doses combined is shown in green and used as benchmark, and the ROC surface for each individual formulation is shown in red, green and blue representing values on the z-axis lower than, equivalent to and higher than the benchmark ROC surface. ELISPOT, enzyme-linked immunospot; GLA-SE, glucopyranosyl lipid adjuvant-stable emulsion; IgG, immunoglobulin G; MN, microneutralizing antibody; PCA, palivizumab-competitive antibody; ROC, receiver-operating characteristic; ROC-P, receiver-operating characteristic of postdose levels; VUS, volume under the surface.