Figure 1.

Construction and characterization of MnFe₂O₄ NPs coated with CMX. (A) DLS results of citrate-coated MnFe₂O₄ NPs (Nano, upper panel) and citrate-coated MnFe₂O₄ NPs incubated with CMX (NCMX, lower panel). (B) TEM images of citrate-coated MnFe₂O₄ NPs. Characterization of CMX protein corona formation of Nano (C) and NCMX (D). (E) NCMX samples were separated by lateral magnetic separation and utilized for the dot plot. The first dot represents only citrate-coated Nano; the second dot represents only CMX as a positive control; the third dot represents magnetically separated citrate-coated Nano covered with CMX (NCMX); and the last dot is the residual protein in the supernatant after the adsorption of CMX onto the NPs (residual CMX). (F) Quantification of CMX adsorbed onto the NPs. NCMX was incubated with 10% SDS and then centrifuged, and the supernatant left was used for western blotting and comparison with a known concentration of CMX.