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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2018 Nov 14;1866(2):252–263. doi: 10.1016/j.bbamcr.2018.11.005

Fig. 5.

Fig. 5

Effect of co-transfection with dominant negative mutants of SEC24. HEK293 cells were either transfected with p3xFLAG-CMV14/B0,+ vector or co-transfected with SEC24C-VN or SEC24D-VN, at ratio indicated in the figure. (A) Level of SEC24 proteins was analyzed by Western blot with the corresponding anti-SEC24 antibodies. Level of over-expressed ATB0,+ was analyzed with anti-FLAG antibodies. (B) The cells were biotinylated with membrane impermeable reagent and the biotinylated fraction, as well as total protein content were analyzed by Western blot with anti-FLAG antibodies. GAPDH was detected, as loading control.