Figure 5.

Ablation or acute inhibition of PPG neurons increases food intake only after a large meal. Cumulative (A and B) and noncumulative (C) food intake of control and PPG-ablated mice in the first 4 h (A and C) and 21 h (B) after the onset of the dark phase after 18 h of food deprivation prior to the onset of dark. Data are given as the mean ± SEM; n = 7 (control), n = 6 (DTA). A: No significant virus × time interaction (F(2,22) = 1.81, P = 0.19), but a significant main effect of virus (F(1,11) = 8.0, P = 0.016). B: P = 0.056 (unpaired t test). C: No significant virus × time interaction (F(2,22) = 0.75, P = 0.49), but a significant main effect of virus (F(1,11) = 6.1, P = 0.031). Cumulative (D and E) and noncumulative (F) food intake of hM₄Di-expressing mice injected with saline or CNO (2 mg/kg i.p.; 30 min prior to dark onset) in the first 4 h of the dark phase after 18 h of food deprivation prior to the onset of dark. Data are given as the mean ± SEM; n = 12. D: There was a significant virus × drug interaction at hour 1 (F(1,21) = 4.733, P = 0.0411), P = 0.038 (CNO vs. saline, Sidak multiple comparisons test). E: No significant virus × drug interaction (F(1,21) = 1.245, P = 0.28) and no significant main effect of drug (F(1,21) = 1.67, P = 0.21) or virus (F(1,21) = 0.084, P = 0.77). F: There was a significant virus × drug interaction at hour 1 (F(1,21) = 4.733, P = 0.0411), P = 0.038 (CNO vs. saline; Sidak multiple-comparisons test). Data from mice expressing control virus are included in analysis. *P < 0.05. n.s., not significant.