Table 1.
Sources of virus and antibody preparations used
| Virus/antibody; titer/dilution | Application | Source | References |
|---|---|---|---|
| AAV2-FLEX-hM3Dq:mCherry; 6.1 × 1012 | Activation of Cre-expressing PPG neurons | UNC Vector Core, Chapel Hill, NC | pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (49) |
| AAV8-FLEX-hM3Dq:mCherry; 4 × 1012 | Activation of Cre-expressing PPG neurons | VVF, ZNZ, Zurich, Switzerland | pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (49) |
| AAV8-mCherry-FLEX-DTA; 3.3 × 1012 | Ablation of Cre-expressing PPG neurons | UNC Vector Core, Chapel Hill, NC | pAAV-mCherry-flex-dtA was a gift from Naoshige Uchida |
| AAV2-FLEX-hM4Di:mCherry; 6.4 × 1012 | Inhibition of Cre-expressing PPG neurons | VVF, ZNZ, Zurich, Switzerland | pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (49) |
| AAV1/2-FLEX-Perceval; titer not determined | Control for viral transduction | Made in-house | pAAV-FLEX-empty was a gift from Bill Wisden (50) pShuttleCMV-Perceval was a gift from Guy Rutter |
| AAV8-FLEX-EGFP; 8 × 1012 | Control for viral transduction | VVF, ZNZ, Zurich, Switzerland | pAAV-hSyn-DIO-EGFP was a gift from Bryan Roth |
| PRSx8-AlstR-EGFP-LV; 1 × 1010 | Identification of Phox2b neurons | Sergei Kasparov | (26) |
| Chicken anti-GFP, Alexa Fluor 488 goat anti-chicken; 1:1,000 | GCaMP3, EGFP, YFP | AB13970; Abcam/#A-11039; Invitrogen | (18) |
| Rabbit anti-dsRed, Cy3 sheep anti-rabbit; 1:1,000 | mCherry, tdRFP | #632496; TaKaRa Bio Inc./#C2306; Sigma | |
| Rabbit anti-cFOS; 1:500 Alexa Fluor 488 goat anti-rabbit; 1:1,000 | cFOS | #ABE457; Merck/#A-11008; Invitrogen |
UNC, University of North Carolina; VVF, Viral Vector Facility; ZNZ, Neuroscience Center Zurich.