Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 7;216(1):176–194. doi: 10.1084/jem.20181170

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Lee et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Omental metastasis is decreased in NET-defective, neutrophil-specific, Padi4-deficient mice. Female neutrophil-specific Padi4^−/− mice and control siblings were injected i.b. with ID8 cells that express RFP and luciferase and thereafter were evaluated for NET formation and tumor progression. (A) At 3 wk, mice in both groups were euthanized (n = 6 mice per group). Fresh omental tissues were stained with Ly6G Ab and Sytox Red dye, and staining was analyzed by flow cytometry. Shown is the proportion of omental Ly6G⁺ cells that were Sytox Red⁺ in each mouse of each group. ***, P < 0.001 (unpaired t test). (B and C) Representative examples of primary tumors detected at 3 wk by bioluminescence imaging (arrowheads; B) and by light microscopy (circled; C). Bar, 10 mm. (D–G) Analysis of tumor progression at 6 wk (n = 6 mice per group). (D) Sizes of primary tumors and omental implants detected in each mouse by bioluminescence imaging. **, P < 0.01 (unpaired t test). (E) Representative images of mice with primary tumors (arrowheads) and omental implants (arrows). (F) Representative images of tumors viewed under light microscopy (circled). Bars, 10 mm. (G) Abundance of RFP⁺ tumor cells in tissues of the primary site, visceral fat tissues, and peritoneal fluid in each mouse, quantified by flow cytometric analysis. *, P < 0.05; ***, P < 0.001 (unpaired t test). Error bars in A, D, and G represent SD.