Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 7;218(1):190–205. doi: 10.1083/jcb.201805047

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Lindeboom et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Analysis of microtubule crossovers in +TIP mutants. (A and B) Examples of crossovers (blue arrowhead) where severing occurs, followed by depolymerization (A; see Video 2) or polymerization (B; see Video 3) of the new plus end (yellow arrowhead) in a WT cell expressing YFP-TUA5. Scale bars are 3 µm. (C) Severing probability per crossover in cells expressing YPF-TUA5 in WT, spr1, 3x-eb1, and clasp backgrounds (n = 2,027, 1,696, 1,718, and 1,147 crossovers in six cells, respectively). Error bars show 95% confidence internals. Asterisks indicate a significant difference from WT by Fisher’s exact test. (D) Waiting times from the observed moment of crossover generation until observed evidence for MT severing in WT, spr1, 3x-eb1, and clasp backgrounds (n = 2,027, 1,696, 1,718, and 1,147 crossovers in six cells, respectively). Boxplots show the 25th and 75th percentile as box edges, the line in the box indicates median value, and the whiskers show the 2.5th and 97.5th percentile. A Kruskal–Wallis test showed significant differences in sever waiting times among the genotypes (P < 0.001). Asterisks indicate significant difference from WT by Mann–Whitney U test. (E) Probability of new plus ends created by severing at crossovers being initially observed in a growing state (n = 882, 679, 764, and 598 crossover severing events in WT, spr1, 3x-eb1, and clasp backgrounds, respectively). Error bars show 95% confidence intervals. Asterisks indicate a significant difference from WT by Fisher’s exact test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.