Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 7;218(1):299–316. doi: 10.1083/jcb.201802076

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Kruse et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — N-cadherin–activated signaling restricts junctional permeability to albumin. (A) Confocal live cell imaging of HPAECs expressing VE-cadherin–GFP (green) showing albumin–Alexa Fluor 647 (red) permeation across AJs. x-y (left) and x-z (enlarged, shown over time) sectional area of VE-cadherin–GFP junction. Albumin–Alexa Fluor 647 was added apically at 5 min. Time is shown in minutes; bar, 5 µm. (B) Measurement of average fluorescent intensity of albumin–Alexa Fluor 647 within VE-cadherin–GFP junction over time. Fluorescence was normalized to starting fluorescent intensity before addition of albumin–Alexa Fluor 647. (C) Bar graph showing the permeability rate constant from B in endothelial monolayers on gelatin and N-cad-BioS. The permeability rate constant was reduced in cells grown on N-cad-BioS as compared with gelatin. n = 14–15 junctions from three independent experiments. (B) Data are presented as mean ± SEM; (C) data are presented as mean ± SD; *, P < 0.05, a two-tailed, unpaired t test.