Table 1. Oligonucleotide primers for PCR amplification of candidate genes.
Candidate Enzyme | Forward Primer | Reverse Primer | Restriction Endonucleases |
---|---|---|---|
β-glucosidase | GAATTCAAGGATCCAATGAACAAGACCCAC | AAAAAACTGCAGCATAGCGGCCGCTGAGCTCTTAAGCTT | BamHI/NotI |
α-L-arabinofuranosidase | GAATTCTGGATCCAATGGGTCTTGCTATCG | AAAAAACTGCAGCATAGCGGCCGCTGAGCTCTTCTGAG | BamHI/NotI |
β-xylosidase | AAAAAAGGATCCTATGGCTAACCCGTACTTACCTG | AAAAAAGAGCTCATTACTTGGTAAACACG AACGCC | BamHI/SacI |
endo-1,4-β-xylanase | GAATTCTGGATCCAATGATCAATAAATTACTGTCATCGGCATTGGCA | AAAAAACTGCAGCATAGCGGCCGCTGAGCTCTTAAGCTT | BamHI/NotI |