. 2019 Jan 2;14(1):e0208606. doi: 10.1371/journal.pone.0208606

Table 1. Primers used for DNA amplification and sequencing of mitochondrial loci.

Target	Primer	Primer sequence(5’-3’)	Amplicon size (bp)	Annealing Temperature (°C)
Region 1 rp15-rns	PiR1-F	`AAGA TATACCTCCAATAGATG` 3,985–4,005¹	Haplotype I—1.3 kb Haplotype II—3.2 kb	56
	PiR1-R	`GATAATTCACGTGTTACTCAC` 5,308–5,288
	PiR1-Fn1²	`GTT TTATTTAAACCTTCTTCAG`
	PiR1-Fn2²	`CTCATA TTTTGAATAAAGTCTC`
	PiR1-Fn3²	`TTATATAACCAAAAACTACT TC`
	PiR1-Rn1²	`ACCAAGATCAAATATTAGATCA`
	PiR1-Rn2²	`AAGTTAGGACATATTTACATGA`
	PiR1-Rn3²	`AAATTATCAAATAGGTAAACGAA`
Region 2 rns- cox2	PiR2-F	`AATTAGTAACT TTGATGAAGT C` 6,638–6,659	1.1 kb	60
Region 2 rns- cox2	PiR2-R	`AAACCTAATTGCCAAGGTTC` 7,731–7,712	1.1 kb
Region 3 cox1-nad9	PiR3-F	`AAATGTTACCTTTTTTCCAATG` 9,925–9,946	0.85 kb	56
Region 3 cox1-nad9	PiR3-R	`ATAGGAATTAATTTATCTGAAG` 10,770–10,749	0.85 kb
Region 4 nad3-nad5	PiR4-F	`CATGGGGATTTTGGACTATG` 22,745–22,764	0.4 kb	56
Region 4 nad3-nad5	PiR4-R	`AAATACCGAACCTTTACGAC` 23,145–23,126	0.4 kb
Region 5 nad6-nad4L	PiR5-F	`CCTTAATAGGTGCAGTAACT` 25,674–25,693	0.5 kb	62
Region 5 nad6-nad4L	PiR5-R	`GTAGCAGCAGCAGAATCTG` 26,166–26,148	0.5 kb

¹ Base position in P. infestans mitochondrial genome NC002387

² Nested primers used for sequencing of region 1.