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. Author manuscript; available in PMC: 2019 Dec 19.
Published in final edited form as: Neuron. 2018 Nov 8;100(6):1474–1490.e4. doi: 10.1016/j.neuron.2018.10.027

Figure 6: AIP neurons are sufficient and necessary for natural threat displays.

Figure 6:

A) Representative Chrimson::tDT expression from a “triple” intersection (top) between SplitThr (bottom left) and R21B10 (Gal4, bottom right) with AIP (arrowheads) and dorsal neurons (arrows) indicated. B) This intersection labels AIP neurons (i, ii), not (mostly) dorsal neurons (iii), and promotes light induced threats (iv). Grey box (ii) indicates UAS-Chrimson not FLP-ON. C) Chrimson::tDT expression (i) in 3 AIP neurons in a fly that exhibited a light induced, multi element threat (ii; SplitThr2). D) Total behaviors per pair (left) according to genotype. (Significance from pairwise and multiple comparisons tests as indicated). Representative brain expression (right) for SplitThr (i), SplitThr3 (ii), and a triple intersection (iii) with AIP (arrowheads) and dorsal neurons (arrows) indicated. Scale bars (A, C, D) are 50μm.