KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-DsRed (rabbit) | Clontech | Cat#632496 RRID:AB_10013483 |
| nc82 (mouse) | Developmental Studies Hybridoma Bank | RRID:A2314866 |
| anti-GFP (mouse) | Thermo Fisher Scientific | Cat#A11120 RRID:AB_221568 |
| anti-GFP (rabbit) | Thermo Fisher Scientific | Cat#A11122 RRID:AB_221569 |
| Goat anti-mouse Alexa Flour 488 | Thermo Fisher Scientific | Cat#A11001 RRID:AB_2534069 |
| Goat anti-rabbit Alexa Flour 488 | Thermo Fisher Scientific | Cat#A11008 RRID:AB_143165 |
| Goat anti-rabbit Alexa Flour 568 | Thermo Fisher Scientific | Cat#A11011 RRID:AB_143157 |
| Goat anti-mouse Alexa Flour 633 | Thermo Fisher Scientific | Cat#A21050 RRID:AB_141431 |
| Rabbit anti-FruM | Barry Dickson | N/A |
| Rat anti N-cadherin | Developmental Studies Hybridoma Bank | RRID:AB221568 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| All trans-Retinal | Sigma-Aldrich | Cat#R2500 |
| Insect-A-Slip | BioQuip Products | Cat#2871B |
| Experimental Models: Organisms/Strains | ||
| Drosophila: Wild-type CantonS | M. Heisenberg | N/A |
|
Drosophila: Quad olfactory mutant IR8a, IR25a, OR83b, Gr63a |
Ana Silbering, Richard Benton | N/A |
| Drosophila: R20E08-Gal4 (attp2) | Rubin lab | RRID:BDSC_69771 |
| Drosophila: pBDP-Gal4U “empty” (attp2) | Rubin lab | N/A |
| Drosophila: Otd-nls::FLPo (attp40) | K. Watanabe | N/A |
| Drosophila: 20×UAS>tdTomato>dTrpA1 (VK5) | Rubin lab | N/A |
| Drosophila: 20×UAS>dTrpA1>tdTomato (VK5) | Rubin lab | N/A |
| Drosophila: 10×UAS >mCherry>Kir 2.1::GFP (attp2) | K. Watanabe | N/A |
| Drosophila: 10× UAS>Kir 2.1::GFP>mCherry (attp2) | K. Watanabe | N/A |
| Drosophila: 10×UAS-IVS-myr::GFP (attp5) | Rubin lab | N/A |
| Drosophila: R22D03-AD (attp40) | Rubin lab | N/A |
| Drosophila: R20E08-DBD (attp2) | Rubin lab | N/A |
| Drosophila: UAS-dTrpA1 | P. Garrity | N/A |
| Drosophila: 20×UAS-IVS-Syn21-Chrimson::tdT-3.1 (VK5) | Rubin lab (this study) | N/A |
| Drosophila: norpA36 | Bloomington Drosophila Stock Center | 9048 RRID:BDSC_9048 |
| Drosophila: R21B10-Gal4 (attp2) | Rubin lab | RRID: BDSC_49295 |
| Drosophila: R21B10-FLP | H. Inagaki | N/A |
| Drosophila: 20×UAS-FRT> myr::TopHat2->FRT-Syn21-Chrimson::tdT 3.1 (VK5) | Rubin lab (this study) | N/A |
| Drosophila: R20E08-AD (attp40) | Rubin lab | N/A |
| Drosophila: R34H05-DBD (attp2) | Rubin lab | N/A |
| Drosophila: R22D03-DBD (attp2) | Rubin lab | N/A |
| Drosophila: pBDP-AD “empty” (attp2) | Rubin lab | N/A |
| Drosophila: pBDP-DBD “empty” (attp2) | Rubin lab | N/A |
| Drosophila: 10×UAS-DenMark (attp40) | Rubin lab | N/A |
| Drosophila: 3×UAS-IVS-syt-GFP (attp18) | Rubin lab | N/A |
| Drosophila: 20E08-LexA (attp40) | Rubin lab | N/A |
| Drosophila: 13×LexAOP-GFP (attp8) | Rubin lab | N/A |
| Drosophila: R11F03-DBD (attp2); R71D08-AD (VK27) | Y. Aso, Rubin lab | N/A |
| Drosophila: 10×UAS-tdT (attp18) | Rubin lab | N/A |
| Software and Algorithms | ||
| Bias video acquisition | Will Dickson, IORodeo | N/A |
| Caltech Fly Tracker | Eyrun Eyjolfsdottir and Pietro Perona | N/A |
| Janelia Automatic Animal Behavior Annotator (JAABA) | Kristen Branson, Janelia Research Campus | N/A |
| Matlab R2013b | Mathworks | RRID: SCR_001622 |
| Fiji | https://fiji.sc/ | RRID: SCR_002285 |