Table 2.
Recovery of targeted oxylipins from quality control samples at five different concentration levels (n = 3 or 4)
| Compound | LQC2 (0.5 ng/mL)* | LQC2 (0.5 ng/mL)* | QC3 (1 ng/mL)* | QC4 (10 ng/mL)* | QC5 (80 ng/mL)* | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PMC | Plasma | Plasma | PMC | Plasma | PMC | Plasma | PMC | Plasma | ||||||||||
| RC | RSD% | RC | RSD% | RC | RSD% | RC | RSD% | RC | RSD% | RC | RSD% | RC | RSD% | RC | RSD% | RC | RSD% | |
| 9-HODE | 98.4 | 1.8 | 89.8 | 15.1 | 93.3 | 13.4 | 98.9 | 12.4 | 95.1 | 7.7 | 93.4 | 5.6 | 99.6 | 10.5 | 102.6 | 4.6 | 88.4 | 4.8 |
| 13-HODE | NQ | NA | NQ | NA | 97.3 | 11.0 | 103.5 | 6.8 | 99.9 | 14.5 | 94.9 | 7.8 | 104.0 | 4.1 | 103.7 | 7.5 | 87.1 | 9.3 |
| 9-oxoODE | 109.5 | 17.0 | 87.3 | 8.2 | 95.9 | 7.7 | 102.8 | 15.7 | 99.4 | 9.8 | 99.3 | 2.2 | 101.7 | 11.9 | 103.9 | 11.7 | 80.8 | 14.3 |
| 13-oxoODE | 90.1 | 21.9 | 103.2 | 10.9 | 99.6 | 11.1 | 101.7 | 14.0 | 89.6 | 15.1 | 98.1 | 3.7 | 100.0 | 10.1 | 104.6 | 11.5 | 82.0 | 11.0 |
| 9,10-EpOME | NQ | NA | NQ | NA | NQ | NA | 96.0 | 7.9 | 114.2 | 19.2 | 92.5 | 2.6 | 101.7 | 12.7 | 104.1 | 8.8 | 86.0 | 4.0 |
| 12,13-EPOME | NQ | NA | NQ | NA | NQ | NA | 99.4 | 15.2 | 101.9 | 13.4 | 91.9 | 2.9 | 100.0 | 18.1 | 103.0 | 10.4 | 83.8 | 8.3 |
| 9,10-DiHOME | 102.7 | 4.6 | 82.4 | 12.9 | 90.7 | 10.4 | 102.6 | 7.3 | 94.6 | 4.9 | 100.9 | 7.2 | 97.7 | 13.5 | 107.9 | 3.8 | 88.9 | 10.2 |
| 12,13-DiHOME | 99.8 | 7.4 | 90.3 | 12.6 | 73.3 | 12.0 | 101.3 | 10.7 | 90.1 | 6.8 | 99.8 | 5.6 | 99.6 | 15.0 | 108.6 | 6.3 | 88.1 | 7.5 |
| 9,12,13-TriHOME | NQ | NA | NQ | NA | NQ | NA | 100.8 | 2.5 | 112.6 | 13.9 | 100.4 | 3.2 | 102.6 | 13.1 | 105.5 | 8.1 | 89.1 | 11.0 |
| 9,10,13-TriHOME | NQ | NA | NQ | NA | 100.4 | 9.9 | 101.0 | 7.4 | 100.7 | 9.2 | 99.5 | 4.0 | 101.0 | 12.3 | 104.9 | 6.0 | 90.7 | 9.9 |
| 9,10,11-TriHOME | 116.2 | 8.3 | 97.0 | 10.6 | 98.6 | 11.4 | 96.5 | 5.0 | 94.8 | 14.1 | 99.8 | 5.4 | 100.0 | 11.0 | 105.0 | 11.0 | 90.8 | 12.9 |
| 9-HOTrE | 96.3 | 1.6 | 97.1 | 5.7 | 95.4 | 7.1 | 102.4 | 10.9 | 98.2 | 11.0 | 96.8 | 10.0 | 99.0 | 7.8 | 103.3 | 7.1 | 88.9 | 4.2 |
| 13-HOTrE | 107.0 | 3.4 | 79.8 | 25.5 | 85.6 | 5.1 | 101.1 | 14.8 | 98.1 | 9.4 | 96.8 | 7.1 | 99.8 | 12.1 | 106.1 | 6.7 | 84.9 | 12.1 |
| PGE2 | 102.4 | 10.7 | 92.5 | 14.0 | 104.9 | 6.7 | 107.9 | 6.7 | 97.1 | 13.8 | 104.5 | 9.3 | 100.9 | 7.0 | 113.4 | 7.7 | 93.1 | 10.1 |
| PGF2α | NQ | NA | NQ | NA | NQ | NA | 103.7 | 8.5 | 92.9 | 7.5 | 99.0 | 8.9 | 98.1 | 8.1 | 104.8 | 8.9 | 89.1 | 13.1 |
| 8-IsoPGF2α | NQ | NA | NQ | NA | 104.6 | 12.3 | 107.7 | 6.0 | 94.8 | 10.7 | 100.6 | 6.7 | 101.6 | 10.4 | 105.2 | 8.2 | 89.4 | 12.7 |
| TXB2 | 95.6 | 4.7 | 102.5 | 5.2 | 97.0 | 4.0 | 101.8 | 9.3 | 95.1 | 12.0 | 99.8 | 4.4 | 97.4 | 13.6 | 103.5 | 13.7 | 89.4 | 12.6 |
| LTB4 | 90.7 | 5.1 | 110.4 | 10.4 | 94.4 | 10.4 | 100.2 | 9.0 | 96.4 | 5.4 | 97.1 | 2.7 | 97.3 | 13.0 | 104.7 | 7.7 | 85.8 | 13.9 |
| LXA4 | 98.6 | 9.6 | 69.6 | 7.4 | 92.1 | 6.3 | 102.8 | 9.7 | 93.8 | 6.3 | 97.4 | 6.5 | 98.3 | 9.6 | 107.5 | 7.6 | 87.9 | 14.9 |
| LXB4 | 104.1 | 13.3 | NQ | NA | 98.8 | 10.8 | 102.6 | 8.9 | 96.9 | 2.1 | 99.0 | 5.5 | 98.9 | 8.5 | 104.2 | 6.2 | 87.9 | 15.0 |
| 5-HETE | 86.4 | 4.2 | 85.1 | 13.5 | 92.3 | 1.8 | 89.0 | 11.5 | 96.2 | 12.4 | 86.7 | 2.0 | 98.3 | 10.3 | 97.4 | 8.9 | 84.1 | 12.5 |
| 8-HETE | 96.5 | 7.6 | 104.3 | 12.1 | 92.6 | 7.0 | 89.5 | 8.3 | 90.6 | 7.9 | 87.9 | 4.9 | 98.2 | 9.4 | 100.9 | 12.9 | 81.2 | 12.8 |
| 9-HETE | 93.5 | 13.0 | 91.9 | 11.7 | 94.9 | 8.6 | 97.0 | 7.8 | 95.1 | 13.1 | 89.0 | 6.8 | 99.6 | 10.3 | 99.4 | 14.1 | 83.3 | 7.0 |
| 11-HETE | 94.1 | 1.9 | 100.1 | 9.8 | 92.7 | 10.5 | 92.9 | 11.7 | 95.2 | 11.1 | 89.7 | 2.4 | 99.3 | 12.6 | 102.1 | 12.1 | 85.0 | 9.5 |
| 12-HETE | 90.9 | 15.6 | 88.2 | 13.9 | 85.7 | 5.7 | 96.4 | 12.5 | 96.1 | 12.0 | 94.5 | 3.0 | 99.1 | 9.2 | 101.6 | 11.7 | 81.6 | 12.0 |
| 15-HETE | 96.2 | 9.6 | 95.3 | 17.9 | 96.9 | 9.7 | 89.6 | 14.8 | 101.1 | 6.7 | 92.0 | 4.3 | 102.4 | 7.7 | 104.0 | 12.9 | 84.9 | 11.7 |
| 20-HETE | 121.2 | 21.8 | 86.7 | 10.7 | 96.7 | 9.4 | 103.0 | 12.8 | 103.6 | 17.4 | 94.0 | 12.2 | 103.6 | 3.0 | 104.0 | 11.2 | 83.6 | 15.6 |
| 5-oxoETE | 85.2 | 10.2 | 87.4 | 12.7 | 95.5 | 8.5 | 86.3 | 12.7 | 97.2 | 12.1 | 99.0 | 11.2 | 96.5 | 18.5 | 99.9 | 7.8 | 88.8 | 10.8 |
| 5,6-EET | − | − | − | − | − | − | 38.3 | 13.4 | 61.3 | 11.7 | 43.4 | 5.1 | 66.2 | 7.9 | 53.1 | 13.8 | 63.3 | 13.8 |
| 8.9-EET | 89.3 | 5.0 | 89.8 | 9.6 | 97.3 | 8.8 | 86.9 | 7.4 | 97.0 | 10.1 | 83.0 | 2.9 | 97.4 | 12.2 | 95.8 | 10.2 | 79.3 | 11.6 |
| 11,12-EET | 82.3 | 12.0 | 94.4 | 4.8 | 91.6 | 6.9 | 91.5 | 5.1 | 93.7 | 5.8 | 81.9 | 4.7 | 95.6 | 10.7 | 96.0 | 9.4 | 81.6 | 11.8 |
| 14,15-EET | 92.1 | 7.4 | 93.7 | 3.0 | 97.9 | 5.7 | 92.0 | 13.2 | 92.7 | 5.8 | 90.9 | 6.9 | 99.7 | 12.0 | 99.5 | 5.7 | 86.1 | 12.6 |
| 18-HEPE | 98.6 | 7.6 | 99.8 | 6.3 | 92.4 | 6.1 | 98.0 | 12.1 | 98.2 | 10.7 | 95.3 | 6.4 | 98.5 | 11.3 | 101.9 | 7.3 | 84.8 | 10.6 |
| 14,15-EEQ | 93.6 | 4.9 | 92.3 | 7.9 | 93.6 | 7.7 | 95.8 | 7.9 | 92.0 | 9.5 | 91.7 | 5.9 | 100.5 | 11.3 | 103.1 | 12.7 | 82.7 | 10.9 |
| 17,18-EEQ | 103.8 | 5.8 | 103.8 | 11.1 | 91.8 | 8.1 | 104.6 | 14.1 | 96.8 | 5.8 | 94.8 | 2.7 | 100.4 | 9.2 | 104.6 | 11.9 | 80.4 | 14.8 |
| 4-HDHA | 87.8 | 5.5 | 109.5 | 15.4 | 92.3 | 10.7 | 86.3 | 10.9 | 96.3 | 6.5 | 77.7 | 2.7 | 95.5 | 10.7 | 90.7 | 13.5 | 83.6 | 10.6 |
| 7-HDHA | 101.0 | 9.8 | 99.5 | 7.2 | 95.7 | 5.9 | 94.6 | 6.7 | 97.2 | 13.6 | 86.0 | 5.9 | 98.3 | 11.3 | 97.7 | 11.5 | 83.4 | 11.5 |
| 10-HDHA | 88.2 | 6.8 | 95.2 | 6.2 | 91.6 | 14.6 | 86.0 | 6.9 | 97.5 | 13.6 | 88.2 | 5.4 | 98.3 | 10.4 | 98.8 | 13.2 | 83.0 | 10.8 |
| 14-HDHA | 106.7 | 17.8 | 92.5 | 17.4 | 93.2 | 7.2 | 90.5 | 11.4 | 99.4 | 9.7 | 85.1 | 4.9 | 98.3 | 12.6 | 98.3 | 12.5 | 82.2 | 12.7 |
| 17-HDHA | 93.8 | 14.1 | 73.7 | 11.4 | 88.1 | 12.7 | 99.5 | 8.9 | 104.2 | 11.8 | 88.3 | 2.1 | 104.1 | 7.7 | 98.4 | 11.9 | 86.3 | 12.7 |
| PDX | 97.9 | 6.3 | 100.8 | 10.2 | 96.1 | 8.9 | 98.0 | 11.3 | 96.7 | 4.5 | 96.5 | 3.9 | 98.7 | 12.1 | 107.0 | 9.9 | 85.9 | 12.3 |
| MaR1 | 89.2 | 5.7 | 93.4 | 2.3 | 91.0 | 4.9 | 100.3 | 13.7 | 95.4 | 5.5 | 96.2 | 6.3 | 96.1 | 11.3 | 107.1 | 7.9 | 86.8 | 12.1 |
| RvD1 | 92.4 | 8.8 | 72.1 | 4.8 | 93.2 | 10.1 | 98.8 | 15.7 | 87.3 | 11.3 | 101.6 | 4.0 | 100.0 | 7.3 | 103.0 | 9.6 | 90.8 | 5.3 |
| RvD2 | NQ | NA | NQ | NA | 97.2 | 14.3 | 87.9 | 15.0 | 100.0 | 5.5 | 98.5 | 6.6 | 97.4 | 7.7 | 102.1 | 7.7 | 88.4 | 13.3 |
| 16,17-EDP | 86.4 | 7.9 | 88.5 | 13.7 | 82.9 | 9.2 | 81.9 | 7.2 | 103.3 | 15.0 | 84.6 | 4.0 | 101.3 | 15.8 | 94.6 | 11.5 | 80.5 | 9.9 |
| 19,20-EDP | 91.6 | 8.3 | 97.4 | 10.8 | 87.1 | 8.2 | 81.2 | 12.1 | 99.1 | 6.8 | 83.4 | 5.1 | 98.3 | 10.9 | 96.1 | 10.3 | 79.5 | 14.1 |
| 11-H-9-E-LA | NQ | NA | NQ | NA | 99.3 | 16.1 | 92.4 | 13.9 | 80.1 | 17.2 | 101.3 | 6.6 | 100.3 | 14.0 | 108.4 | 8.1 | 87.8 | 12.8 |
| 13-H-9-E-LA | 104.7 | 17.7 | 91.0 | 9.7 | 91.7 | 14.9 | 91.0 | 20.0 | 94.6 | 8.4 | 102.1 | 7.2 | 98.5 | 13.5 | 107.1 | 10.2 | 86.9 | 13.1 |
| 9-H-12-E-LA | 111.8 | 7.4 | 99.3 | 11.4 | 86.2 | 14.2 | 94.4 | 18.3 | 92.3 | 8.4 | 102.4 | 5.0 | 96.0 | 12.3 | 108.4 | 7.4 | 86.7 | 14.5 |
| 11-H-12-E-LA | NQ | NA | NQ | NA | NQ | NA | 71.3 | 20.3 | 66.8 | 10.4 | 104.3 | 4.7 | 95.0 | 13.6 | 107.8 | 8.6 | 92.0 | 14.0 |
Recoveries (RC) of oxylipins were evaluated using four or five spiked concentrations for PMC and plasma, respectively, and oxylipin levels in plasma were adjusted for endogenous level subtraction. Experimental details in Section 1.9.5. For EHODEs and EKODEs with two or three peaks, recoveries were determined by sum of each peaks in Excel. NQ: the concentration is below the LOQ or out of the linear range or not detected, NA: not available,
*
: for 9-HODE and 9-HOTrE, concentration levels of QC1, QC2, QC3, QC4, and QC5 were 2, 5, 10,100, and 800, respectively. Experimental details in Recovery Section under Method