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. Author manuscript; available in PMC: 2019 Jan 2.
Published in final edited form as: Anal Chem. 2015 Jun 11;87(13):6527–6534. doi: 10.1021/acs.analchem.5b01835

Figure 3.

Figure 3.

CE-ESI-TWIM-MS/MS analysis of aminxoyTMT6-128 and aminoxyTMT6-131 differentially labeled LSTa (128/131, 1:4), LSTb (128/ 131, 1:1), LSTc (128/131, 3:1) and DSLNT mixture. (A) EIEs of [LST-aminoxyTMT + H + Na]2+ m/z 661.9 (top) and [DLST-aminoxyTMT + H + 2Na]3+ m/z 545.9 (bottom). The peak at 47.3 min in the top panel corresponds to the in-source fragmentation ion Y4α(Na + H) or Y3β(Na + H) of labeled DSLNT. (B) Arrival time distributions of [LST-aminoxyTMT + H + Na]2+ (m/z 661.9) at a CE migration time of 34.6 min (top), 38 min (middle), and 47.3 min (bottom). (C) Reporter ion ratios of aminoxyTMT-labeled LSTb (top), LSTc (middle), LSTa (bottom) obtained by CEIM-targeted CID MS/MS. The precursor ion [LST-aminoxyTMT + H + Na]2+ (m/z 661.9) was isolated by the quadrupole, separated in the ion mobility drift cell and then subjected to CID MS/MS in the transfer collision cell. CE conditions were as described in Figure 1. Alternating IM-full MS scan and IM-targeted MS/MS scan of m/z 661.9 were acquired during CE separation. TWIM conditions: trap bias voltage = 48 V, helium cell dc = 35 V, wave velocity = 700 m/s, and wave height = 40 V. The transfer cell collision energy was set to 50 V for targeted MS/MS.