Table 1.
LC-MS/MS identification of proteins in the RP-HPLC fractions that contained proteins that bound suLeX in glycan blotsa
| Protein name | General name | Accession | Sequest HT score | MW (kDa) | Coverage (%) | Peptides | Unique peptides | Peptide spectrum matches |
|---|---|---|---|---|---|---|---|---|
| Band B1 (bound suLeX) | ||||||||
| ADAM5 (disintegrin and metalloprotease domain-containing protein 5) |
ADAM5 | E7FM66 | 764.75 | 45.2 | 49.8 | 20 | 20 | 264 |
| Band B2 (did not bind suLeX) | ||||||||
| ADAM3a | – | A5A4F6 | 1330.39 | 82.9 | 22.9 | 19 | 19 | 774 |
| ADAM2 (Fertilin beta) | FTNB | Q866A8 | 413.46 | 81.8 | 14.4 | 11 | 11 | 244 |
| ADAM3b | – | A5HJZ3 | 352.58 | 83.5 | 22.0 | 16 | 16 | 166 |
| Band B3 (bound suLeX) | ||||||||
| Lactadherin | MFGE8 | B2CZF8 | 1773.84 | 47.8 | 60.3 | 37 | 37 | 881 |
a
Proteins from epididymal sperm plasma membrane were identified by LC-MS/MS. Protein bands were excised from a Coomassie-stained one-dimensional gel. Database was searched using the Sequest HT algorithm in Proteome Discoverer.