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. Author manuscript; available in PMC: 2019 Jan 2.
Published in final edited form as: Biochemistry. 2016 Feb 9;55(7):1149–1158. doi: 10.1021/acs.biochem.5b01280

Figure 3.

Figure 3.

O-GlcNAcylation on CTD requires exceptionally long heptad repeats and maximum OGT TPRs. (A) O-GlcNAcylation on a series of SUMO-CTDs containing different numbers of heptads (ranging from 7 to 52 repeats). The glycosylation level was quantified on the basis of [14C]GlcNAc signals via autoradiography and normalized to the same total number of CTD heptads. The data are presented as means ± the standard deviation (SD) (n = 3). (B) O-GlcNAcylation of SUMO-CTD20 by a series of OGTs (varying only in the TPR domain, 4.5, 5.5, 6.5, and 13.5 TPRs). The glycosylation level was quantified on the basis of [14C]GlcNAc signals via autoradiography and normalized to the same concentration of each OGT. The data are presented as means ± SD (n = 3).