Fig. 1.

Transformation of cv.Pusa 9712 half seed explants by Agrobacterium tumefaciens strain EHA105 harboring binary vector pCAMBIA1300-p68 to enhance salinity stress tolerance. a mature dry seeds of soybean cv. Pusa 9712 used for preparing the half seed explants; b 1-day old imbibed seeds; c half seed explants made from imbibed seeds (black arrows direct the embryonic area); d explant after infection with A. tumefaciens EHA105 carrying pCAMBIA1300-p68 plasmid and co-cultivated for 5 days in the dark; e shoot induction from explant in SIM supplemented with cefotaxime (200 mg l^− 1) without NaCl (after 15 days of culture); f, g selection of regenerated shoots in SIM containing cefotaxime (200 mg l^− 1) and NaCl (75 mM) [after 30 days of culture]; h elongated shoots in SEM supplemented with cefotaxime (100 mg l^− 1) and NaCl (75 mM) [after 30 days of culture]; i rooted shoots in RIM supplemented with cefotaxime (100 mg l^− 1) and NaCl (50 mM) [after 30 days of culture]; j putatively transformed (T_o) plants maintained in growth chamber; k acclimatization of survived (T_o) plants under green house condition