Fig. 2. Methylation status and gene expression of ZNF132 in ESCC patients and esophageal cancer cell lines.

a Methylation and gene expression correlation in clinical samples. Y-axis is log-transferred to relative expression similar to that in Fig. 4a; x-axis represents the average methylation level. Dot line indicates the linear regression line. b Expression of ZNF132 measured by q-PCR in ESCC tissues was significantly lower than that in adjacent tissues. c Methylation of ZNF132 in Ec-109 and CaEs-17 cells was significantly reduced after 5-Aza treatment. d Expression of ZNF132 measured by q-PCR significantly increased after 5-Aza treatment. Data are presented as the mean ± S.D. of three independent experiments. *P < 0.05, ***P < 0.001